Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth
1Department of Ophthalmology,Boston Children’s Hospital, 2Department of Ophthalmology,Harvard Tıp Fakültesi, 3F.M. Kirby Neurobiology Center,Boston Children’s Hospital, 4Department of Neurology,Boston Children’s Hospital, 5Department of Neurology,Harvard Tıp Fakültesi, 6Howard Hughes Medical Institute
Bölümler
- 00:04Başlık
- 00:51Pregnancy Confirmation and E10.5 Embryo Harvest and Slice Preparation
- 04:15Results: Representative Ex Vivo Oculomotor Slice Culture Development and Pitfalls
- 05:30Conclusion
An ex vivo slice assay allows oculomotor nerve outgrowth to be imaged in real time. Slices are generated by embedding E10.5 IslMN:GFP embryos in agarose, slicing on a vibratome, and growing in a stage-top incubator. The role of axon guidance pathways is assessed by adding inhibitors to the culture media.
