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Human Blastocyst Biopsy and Vitrification
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Human Blastocyst Biopsy and Vitrification

Human Blastocyst Biopsy and Vitrification

21,484 Views

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10:59 min

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July 26, 2019

DOI:

10.3791/59625-v

DOI:
10.3791/59625-v

•

10:59 min
July 26, 2019

•

30 Views
, , , ,
1G.EN.E.R.A. Centers for Reproductive Medicine, 2DAHFMO, Unit of Histology and Medical Embryology,Sapienza University of Rome

Özet

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Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.

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Maggiulli, R., Giancani, A., Cimadomo, D., Ubaldi, F. M., Rienzi, L. Human Blastocyst Biopsy and Vitrification. J. Vis. Exp. (149), e59625, doi:10.3791/59625 (2019).

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