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Human Blastocyst Biopsy and Vitrification
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July 26, 2019
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Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.
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Maggiulli, R., Giancani, A., Cimadomo, D., Ubaldi, F. M., Rienzi, L. Human Blastocyst Biopsy and Vitrification. J. Vis. Exp. (149), e59625, doi:10.3791/59625 (2019).
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