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An Assay for Quantifying Protein-RNA Binding in Bacteria
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JoVE Journal Genetik
An Assay for Quantifying Protein-RNA Binding in Bacteria

An Assay for Quantifying Protein-RNA Binding in Bacteria

6,452 Views

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07:02 min

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June 12, 2019

DOI:

10.3791/59611-v

DOI:
10.3791/59611-v

•

07:02 min
June 12, 2019

•

5 Views
, , , ,
1Department of Biotechnology and Food Engineering,Technion-Israel Institute of Technology, 2Russell Berrie Nanotechnology Institute,Technion-Israel Institute of Technology

Özet

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In this method, we quantify the binding affinity of RNA binding proteins (RBPs) to cognate and non-cognate binding sites using a simple, live, reporter assay in bacterial cells. The assay is based on repression of a reporter gene.

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Cite this Article

Katz, N., Cohen, R., Atar, O., Goldberg, S., Amit, R. An Assay for Quantifying Protein-RNA Binding in Bacteria. J. Vis. Exp. (148), e59611, doi:10.3791/59611 (2019).

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