このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
An Assay for Quantifying Protein-RNA Binding in Bacteria
6,452 Views
•
07:02 min
•
June 12, 2019
DOI:
概要
Automatically generated
In this method, we quantify the binding affinity of RNA binding proteins (RBPs) to cognate and non-cognate binding sites using a simple, live, reporter assay in bacterial cells. The assay is based on repression of a reporter gene.
Read Article
Cite this Article
Katz, N., Cohen, R., Atar, O., Goldberg, S., Amit, R. An Assay for Quantifying Protein-RNA Binding in Bacteria. J. Vis. Exp. (148), e59611, doi:10.3791/59611 (2019).
Copy