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Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
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Biyokimya
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Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

8,390 Views

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11:13 min

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May 09, 2019

DOI:

10.3791/59573-v

DOI:
10.3791/59573-v

•

11:13 min
May 09, 2019

•

3 Views
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1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry,Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences,University of Applied Sciences Upper Austria

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Here, a quantitative real-time polymerase chain reaction-based protocol is presented for the determination of the native micro-RNA content (absolute/relative) of lipoprotein particles. In addition, a method for increasing the micro-RNA level, as well as a method for determining the cellular uptake rate of lipoprotein particles, is demonstrated.

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Axmann, M., Karner, A., Meier, S. M., Stangl, H., Plochberger, B. Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate. J. Vis. Exp. (147), e59573, doi:10.3791/59573 (2019).

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