ジャーナル
/
生化学
/
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
JoVE Journal
生化学
This content is Free Access.
JoVE Journal 生化学
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

8,407 Views

•

11:13 min

•

May 09, 2019

DOI:

10.3791/59573-v

DOI:
10.3791/59573-v

•

11:13 min
May 09, 2019

•

3 Views
, , , ,
1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry,Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences,University of Applied Sciences Upper Austria

概要

Automatically generated

Here, a quantitative real-time polymerase chain reaction-based protocol is presented for the determination of the native micro-RNA content (absolute/relative) of lipoprotein particles. In addition, a method for increasing the micro-RNA level, as well as a method for determining the cellular uptake rate of lipoprotein particles, is demonstrated.

Explore More Videos

Lipoprotein ParticlesMicroRNACellular UptakeDelipidationEnrichmentRelipidationBradford Protein AssayReconstitutionSpermineSynthetic MicroRNA

Read Article

Cite this Article

Axmann, M., Karner, A., Meier, S. M., Stangl, H., Plochberger, B. Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate. J. Vis. Exp. (147), e59573, doi:10.3791/59573 (2019).

Download .ris file

Copy

Share Video

.

Copy