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生物化学
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Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

8,407 Views

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11:13 min

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May 09, 2019

DOI:

10.3791/59573-v

DOI:
10.3791/59573-v

•

11:13 min
May 09, 2019

•

3 Views
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1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry,Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences,University of Applied Sciences Upper Austria

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Here, a quantitative real-time polymerase chain reaction-based protocol is presented for the determination of the native micro-RNA content (absolute/relative) of lipoprotein particles. In addition, a method for increasing the micro-RNA level, as well as a method for determining the cellular uptake rate of lipoprotein particles, is demonstrated.

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Cite this Article

Axmann, M., Karner, A., Meier, S. M., Stangl, H., Plochberger, B. Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate. J. Vis. Exp. (147), e59573, doi:10.3791/59573 (2019).

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