Dergi
/
İmmünoloji ve Enfeksiyon
/
评估细菌病原体免疫反应的声吞噬杀灭试验
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
Please note that all translations are automatically generated. Click here for the English version.

评估细菌病原体免疫反应的声吞噬杀灭试验

DOI:
10.3791/59400-v

•

08:47 min

•

April 05, 2019

•

, ,
1Department of Biochemistry and Molecular Biology, Center for Molecular Medicine and Complex Carbohydrate Research Center,University of Georgia

Bölümler

  • 00:04Başlık
  • 01:21Culture, Differentiation, and Validation of HL-60 Cells
  • 02:30Preparation of Bacterial Stock Samples
  • 03:53Bacterial Treatment and Culture
  • 04:43HL-60 Bacterial Co-culture
  • 05:46Sample Plating and Overnight Incubation
  • 06:55Results: Determining Bacterial Cell Viability using OPKA
  • 07:55Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

这种光吞噬杀灭方法用于比较吞噬免疫细胞根据不同的处理方法和条件对细菌做出反应和杀灭细菌的能力。传统上, 这种检测方法是评估针对视黄素细菌产生的抗体的效应功能的黄金标准。

Etiketler

Opsonophagocytic Killing AssayImmunotherapeuticAntibacterial ActivityPhagocytosisStreptococcus PneumoniaHL60 CellsBacterial StocksFlow CytometryRPMIFetal Bovine SerumDimethyl SulfoxideDMFBacterial StrainGlycerol

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

酶联免疫斑点法（ELISPOT）：TH - 1细胞对微生物抗原的免疫应答的定量

抗原特异性在体内使用CFSE标记的靶细胞的杀伤含量

单克隆抗体的反对消极行政 H。 capsulatum和其他真菌病原体

“发光法”的分类检测细菌病原体的记者噬菌体

斑马鱼的胚胎细胞内细菌病原体感染

饲养和注射<em>烟草天蛾</em>幼虫评估细菌的毒力

的视觉检测，以监测T6SS介导的细菌竞争

长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用

细菌病原体在细胞和有机水平宿主反应探针中的应用

一种高通量志贺氏菌特异性杀菌试验

Read Article