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Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

8,101 Views

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08:47 min

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March 15, 2019

DOI:

10.3791/59299-v

DOI:
10.3791/59299-v

•

08:47 min
March 15, 2019

•

6 Views
,
1Biyoloji Bölümü,Wesleyan University

Özet

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This paper presents a surgical method for dissecting Drosophila pupal retinas along with protocols for the processing of tissue for immunohistochemistry, western analysis, and RNA-extraction. 

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DrosophilaPupal RetinaImmunohistochemistryWestern AnalysisRNA IsolationDissectionEye-brain ComplexMicrodissectionForcepsPBSFixative

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DeAngelis, M. W., Johnson, R. I. Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation. J. Vis. Exp. (145), e59299, doi:10.3791/59299 (2019).

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