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Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
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発生生物学
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JoVE Journal 発生生物学
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

8,110 Views

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08:47 min

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March 15, 2019

DOI:

10.3791/59299-v

DOI:
10.3791/59299-v

•

08:47 min
March 15, 2019

•

6 Views
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1生物学科,Wesleyan University

概要

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This paper presents a surgical method for dissecting Drosophila pupal retinas along with protocols for the processing of tissue for immunohistochemistry, western analysis, and RNA-extraction. 

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DrosophilaPupal RetinaImmunohistochemistryWestern AnalysisRNA IsolationDissectionEye-brain ComplexMicrodissectionForcepsPBSFixative

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Cite this Article

DeAngelis, M. W., Johnson, R. I. Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation. J. Vis. Exp. (145), e59299, doi:10.3791/59299 (2019).

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