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Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

8,111 Views

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08:47 min

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March 15, 2019

DOI:

10.3791/59299-v

DOI:
10.3791/59299-v

•

08:47 min
March 15, 2019

•

6 Views
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1Department of Biology,Wesleyan University

Summary

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This paper presents a surgical method for dissecting Drosophila pupal retinas along with protocols for the processing of tissue for immunohistochemistry, western analysis, and RNA-extraction. 

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DrosophilaPupal RetinaImmunohistochemistryWestern AnalysisRNA IsolationDissectionEye-brain ComplexMicrodissectionForcepsPBSFixative

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Cite this Article

DeAngelis, M. W., Johnson, R. I. Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation. J. Vis. Exp. (145), e59299, doi:10.3791/59299 (2019).

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