Dergi
/
Biyoloji
/
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

DOI:
10.3791/59178-v

•

10:37 min

•

March 17, 2019

•

, , ,
1Department of Molecular Biology, Cell Biology and Biochemistry,Brown University

Bölümler

  • 00:04Başlık
  • 00:33Sample Collection
  • 01:31Nucleotide and Protein Isolation
  • 07:01Evaluating mRNA with RT-qPCR
  • 08:12Results: Extractions of Different Macromolecules from the Same C. elegans Sample
  • 09:40Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a protocol for the isolation of RNA, DNA, and protein from the same sample, in an effort to reduce variation, improve reproducibility, and facilitate interpretations.

Etiketler

Caenorhabditis ElegansNucleotide ExtractionProtein ExtractionMRNAMacromoleculesSample VariabilityReproducibilitySample CollectionCross-sectional AnalysisWormsM9 BufferGTCP ReagentChloroformRNA IsolationIsopropanolEthanolDNA IsolationProtein Isolation

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4′,6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Read Article