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Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
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JoVE Journal 生物学
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

DOI:
10.3791/59178-v

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10:37 min

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March 17, 2019

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, , ,
1Department of Molecular Biology, Cell Biology and Biochemistry,Brown University

章

  • 00:04標題
  • 00:33Sample Collection
  • 01:31Nucleotide and Protein Isolation
  • 07:01Evaluating mRNA with RT-qPCR
  • 08:12Results: Extractions of Different Macromolecules from the Same C. elegans Sample
  • 09:40Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol for the isolation of RNA, DNA, and protein from the same sample, in an effort to reduce variation, improve reproducibility, and facilitate interpretations.

タグ

Caenorhabditis ElegansNucleotide ExtractionProtein ExtractionMRNAMacromoleculesSample VariabilityReproducibilitySample CollectionCross-sectional AnalysisWormsM9 BufferGTCP ReagentChloroformRNA IsolationIsopropanolEthanolDNA IsolationProtein Isolation

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