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Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans
DOI:

10:37 min

March 17, 2019

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Chapitres

  • 00:04Titre
  • 00:33Sample Collection
  • 01:31Nucleotide and Protein Isolation
  • 07:01Evaluating mRNA with RT-qPCR
  • 08:12Results: Extractions of Different Macromolecules from the Same C. elegans Sample
  • 09:40Conclusion

Summary

Traduction automatique

Here, we present a protocol for the isolation of RNA, DNA, and protein from the same sample, in an effort to reduce variation, improve reproducibility, and facilitate interpretations.

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