Here, we present a highly accessible protocol for evaluating the cell movement in human trophoblast cells using three in vitro assays: the scratch assay, the transwell invasion assay, and the cell proliferation assay.
Kisanga, E. P., Tang, Z., Guller, S., Whirledge, S. In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines. J. Vis. Exp. (145), e58942, doi:10.3791/58942 (2019).