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发育生物学
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In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
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发育生物学
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JoVE 杂志 发育生物学
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

DOI:
10.3791/58942-v

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09:57 min

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March 05, 2019

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, , ,
1Department of Obstetrics, Gynecology and Reproductive Sciences,Yale School of Medicine

Chapters

  • 00:04Title
  • 00:24Scratch Assay
  • 02:05Invasion Assay
  • 06:26Proliferation Assay
  • 08:06Results: Investigating the Role of Glucocorticoids in Trophoblast Cell Migration
  • 09:23Conclusion

Summary

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Here, we present a highly accessible protocol for evaluating the cell movement in human trophoblast cells using three in vitro assays: the scratch assay, the transwell invasion assay, and the cell proliferation assay.

Tags

Keywords: TrophoblastInvasionMigrationProliferationIn Vitro AssayScratch AssayInvasion AssayExtracellular MatrixCell CultureObstetrical Outcomes

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