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Journal / Developmental Biology / In Vitro Assays to Evaluate the Migration,…
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Developmental Biology

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JoVE Journal Developmental Biology
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
 

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Article DOI: 10.3791/58942-v • 09:57 min • March 5th, 2019
March 5th, 2019 •
1, 1, 1, 1
1Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine

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Summary
Automatic Translation

Here, we present a highly accessible protocol for evaluating the cell movement in human trophoblast cells using three in vitro assays: the scratch assay, the transwell invasion assay, and the cell proliferation assay.

Tags

Keywords: Trophoblast Invasion Migration Proliferation In Vitro Assay Scratch Assay Invasion Assay Extracellular Matrix Cell Culture Obstetrical Outcomes
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