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Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

DOI:
10.3791/58806-v

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07:26 min

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January 26, 2019

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, , , , , , , , , , , , , , , ,
1Pediatri Anabilim Dalı,Kobe University Graduate School of Medicine, 2Patoloji Bölümü,Kobe Children’s Hospital, 3Pediatri Anabilim Dalı,Hyogo College of Medicine, 4Department of Developmental Pediatrics,Shizuoka Children’s Hospital, 5Pediatri Anabilim Dalı,Nihon University School of Medicine

'Bölümler'

  • 00:04'Başlık'
  • 01:24Umbilical Cord Dissection
  • 03:08Isolation of Umbilical Cord Mesenchymal Stem Cells (UC-MSCs)
  • 04:16Culture of UC-MSCs
  • 05:42Results: Confirmation of Successful Isolation and Differentiation Capacity of UC-MSCs
  • 06:46Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.

'Etiketler'

Umbilical CordMesenchymal Stem CellsUCMSCPretermTerm InfantsCell TherapyIsolationCharacterizationDissectionEnzymatic DigestionCell CultureProliferation

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