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Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

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07:26 min

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January 26, 2019

DOI:

10.3791/58806-v

DOI:
10.3791/58806-v

•

07:26 min
January 26, 2019

•

12 Views
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1Pediatri Anabilim Dalı,Kobe University Graduate School of Medicine, 2Patoloji Bölümü,Kobe Children’s Hospital, 3Pediatri Anabilim Dalı,Hyogo College of Medicine, 4Department of Developmental Pediatrics,Shizuoka Children’s Hospital, 5Pediatri Anabilim Dalı,Nihon University School of Medicine

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Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.

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Umbilical CordMesenchymal Stem CellsUCMSCPretermTerm InfantsCell TherapyIsolationCharacterizationDissectionEnzymatic DigestionCell CultureProliferation

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Cite this Article

Iwatani, S., Yoshida, M., Yamana, K., Kurokawa, D., Kuroda, J., Thwin, K. K. M., Uemura, S., Takafuji, S., Nino, N., Koda, T., Mizobuchi, M., Nishiyama, M., Fujioka, K., Nagase, H., Morioka, I., Iijima, K., Nishimura, N. Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants. J. Vis. Exp. (143), e58806, doi:10.3791/58806 (2019).

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