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发育生物学
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Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
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JoVE 杂志 发育生物学
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

DOI:
10.3791/58806-v

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07:26 min

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January 26, 2019

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, , , , , , , , , , , , , , , ,
1Department of Pediatrics,Kobe University Graduate School of Medicine, 2Department of Pathology,Kobe Children’s Hospital, 3Department of Pediatrics,Hyogo College of Medicine, 4Department of Developmental Pediatrics,Shizuoka Children’s Hospital, 5Department of Pediatrics,Nihon University School of Medicine

Chapters

  • 00:04Title
  • 01:24Umbilical Cord Dissection
  • 03:08Isolation of Umbilical Cord Mesenchymal Stem Cells (UC-MSCs)
  • 04:16Culture of UC-MSCs
  • 05:42Results: Confirmation of Successful Isolation and Differentiation Capacity of UC-MSCs
  • 06:46Conclusion

Summary

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Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.

Tags

Keywords: Umbilical CordMesenchymal Stem CellsUCMSCPretermTerm InfantsCell TherapyIsolationCharacterizationDissectionEnzymatic DigestionCell CultureProliferation

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