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Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

13,539 Views

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07:26 min

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January 26, 2019

DOI:

10.3791/58806-v

DOI:
10.3791/58806-v

•

07:26 min
January 26, 2019

•

12 Views
, , , , , , , , , , , , , , , ,
1Department of Pediatrics,Kobe University Graduate School of Medicine, 2Department of Pathology,Kobe Children’s Hospital, 3Department of Pediatrics,Hyogo College of Medicine, 4Department of Developmental Pediatrics,Shizuoka Children’s Hospital, 5Department of Pediatrics,Nihon University School of Medicine

Summary

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Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.

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Cite this Article

Iwatani, S., Yoshida, M., Yamana, K., Kurokawa, D., Kuroda, J., Thwin, K. K. M., Uemura, S., Takafuji, S., Nino, N., Koda, T., Mizobuchi, M., Nishiyama, M., Fujioka, K., Nagase, H., Morioka, I., Iijima, K., Nishimura, N. Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants. J. Vis. Exp. (143), e58806, doi:10.3791/58806 (2019).

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