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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
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November 26, 2018
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Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.
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Tedford, K., Tech, L., Steiner, M., Korthals, M., Fischer, K. Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro. J. Vis. Exp. (141), e58759, doi:10.3791/58759 (2018).
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