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Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
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JoVE Journal 免疫学と感染
Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

6,333 Views

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08:31 min

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November 26, 2018

DOI:

10.3791/58759-v

DOI:
10.3791/58759-v

•

08:31 min
November 26, 2018

•

1 Views
, , , ,
1Institute of Biochemistry and Cell Biology,OVGU University of Magdeburg

概要

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Marginal zone B cells (MZBs) respond to the force of shear flow by re-orienting their migration path up the flow. This protocol shows how to record and analyze the migration using a fluidics unit, pump, microscope imaging system, and free software.

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Cite this Article

Tedford, K., Tech, L., Steiner, M., Korthals, M., Fischer, K. Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro. J. Vis. Exp. (141), e58759, doi:10.3791/58759 (2018).

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