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Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

DOI:
10.3791/58471-v

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05:27 min

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January 26, 2019

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, , , , , , , ,
1Department of Molecular and Cellular Biochemistry,Kentucky Üniversitesi, 2Division of Laboratory Animal Resources,Kentucky Üniversitesi, 3River Hill Ranch

Bölümler

  • 00:04Başlık
  • 01:39Generation of Antigen-specific Alpaca Antibodies
  • 03:02Purifying Lymphocytes for Single Domain Antibody Library Construction
  • 03:59Results: Single Domain Antibodies Generated against a Range of Protein Antigens in Alpacas (Lama pacos)
  • 04:46Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A method for the production of single domain antibodies from alpacas, including immunization, blood collection, B-cell isolation, and selection is described.

Etiketler

AlpacaImmunizationProtein AntigensSingle Domain AntibodiesBiomedical ResearchCamelidsAntigen InjectionBlood CollectionHandlingVeterinary Techniques

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