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蛋白质抗原对羊的免疫和抗原特异性单域抗体的制备
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies
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蛋白质抗原对羊的免疫和抗原特异性单域抗体的制备

22,557 Views

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05:27 min

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January 26, 2019

DOI:

10.3791/58471-v

DOI:
10.3791/58471-v

•

05:27 min
January 26, 2019

•

18 Views
, , , , , , , ,
1Department of Molecular and Cellular Biochemistry,Kentucky Üniversitesi, 2Division of Laboratory Animal Resources,Kentucky Üniversitesi, 3River Hill Ranch

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介绍了一种从羊的免疫、血液采集、b 细胞分离和选择等方面制备单域抗体的方法。

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Chow, K. M., Whiteheart, S. W., Smiley, J. R., Sharma, S., Boaz, K., Coleman, M. J., Maynard, A., Hersh, L. B., Vander Kooi, C. W. Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies. J. Vis. Exp. (143), e58471, doi:10.3791/58471 (2019).

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