Immunization of Alpacas (<em>Lama pacos</em>) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

K. Martin Chow; Sidney W. Whiteheart; Jeffrey R. Smiley; Savita Sharma; Kathy Boaz; Meggie J. Coleman; Alvina Maynard; Louis B. Hersh; Craig W. Vander Kooi

doi:10.3791/58471

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Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

JoVE 杂志
生物化学

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JoVE 杂志生物化学

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

22,595 Views

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05:27 min

•

January 26, 2019

DOI:

10.3791/58471-v

DOI:

10.3791/58471-v

•

05:27 min

January 26, 2019

•

18 Views

K. Martin Chow¹, Sidney W. Whiteheart¹, Jeffrey R. Smiley², Savita Sharma¹, Kathy Boaz², Meggie J. Coleman², Alvina Maynard³, Louis B. Hersh¹, Craig W. Vander Kooi¹

¹Department of Molecular and Cellular Biochemistry,University of Kentucky, ²Division of Laboratory Animal Resources,University of Kentucky, ³River Hill Ranch

Summary

A method for the production of single domain antibodies from alpacas, including immunization, blood collection, B-cell isolation, and selection is described.

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Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

Summary

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