We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images.
McCall, A. D., Edgerton, M. Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System. J. Vis. Exp. (140), e58457, doi:10.3791/58457 (2018).