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Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
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JoVE 杂志 免疫与感染
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

DOI:
10.3791/58457-v

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06:16 min

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October 18, 2018

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1Department of Oral Biology,University at Buffalo

Chapters

  • 00:04Title
  • 00:26Preparation of the Flow Apparatus
  • 02:44Imaging with the Flow Apparatus
  • 05:16Results: Candida albicans Biofilm Formation Under Fluid Flow
  • 05:58Conclusion

Summary

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We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images.

Tags

Real-time ImagingFungal BiofilmTwo-phase Recirculating Flow SystemFluid FlowBiofilm Growth And DevelopmentFlow ApparatusBubble TrapTemperature ProbeFilterGrowth FlaskYPD MediaPumpSlide AdapterMicroscope

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