ジャーナル
/
免疫学と感染
/
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

DOI:
10.3791/58457-v

•

06:16 min

•

October 18, 2018

•

,
1Department of Oral Biology,University at Buffalo

章

  • 00:04標題
  • 00:26Preparation of the Flow Apparatus
  • 02:44Imaging with the Flow Apparatus
  • 05:16Results: Candida albicans Biofilm Formation Under Fluid Flow
  • 05:58Conclusion

概要

自動翻訳

自動翻訳

We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images.

タグ

Real-time ImagingFungal BiofilmTwo-phase Recirculating Flow SystemFluid FlowBiofilm Growth And DevelopmentFlow ApparatusBubble TrapTemperature ProbeFilterGrowth FlaskYPD MediaPumpSlide AdapterMicroscope

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Development of a Negative Selectable Marker for Entamoeba histolytica

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Real-time Live Imaging of T-cell Signaling Complex Formation

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Read Article