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无细胞爪卵萃取物微乳液细胞周期振荡的重建
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JoVE Journal Biyokimya
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
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无细胞爪卵萃取物微乳液细胞周期振荡的重建

8,018 Views

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06:31 min

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September 27, 2018

DOI:

10.3791/58240-v

DOI:
10.3791/58240-v

•

06:31 min
September 27, 2018

•

7 Views
, , , ,
1Department of Biophysics,University of Michigan, Ann Arbor, 2Kimya Bölümü,University of Michigan, Ann Arbor, 3Department of Computer Science,Wayne State University, 4Fizik Bölümü,University of Michigan, Ann Arbor

Özet

Automatically generated

本文提出了在油中微乳液中对爪蟾卵提取物进行单细胞水平的体外自持续有丝分裂振荡的生成方法。

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Cell-cycle OscillationsXenopus Egg ExtractsMicroemulsionsCell-free SystemMitotic NetworkCell Cycle ClockHigh-throughputQuantitative ManipulationCell ActivationCell LysisCell Extract Preparation

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Cite this Article

Guan, Y., Wang, S., Jin, M., Xu, H., Yang, Q. Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts. J. Vis. Exp. (139), e58240, doi:10.3791/58240 (2018).

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