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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
Bölümler
- 00:04Başlık
- 01:05Isolation of Rat Kidneys
- 02:17Kidney Tissue Mincing
- 03:18Kidney Tissue Sieving
- 07:12Results: Assessment of Efficient Isolation and Cell Viability of Isolated Glomeruli
- 08:59Conclusion
The main focus of this protocol is to efficiently isolate viable primary glomeruli cultures with minimal contaminants for use in a variety of downstream applications. The isolated glomeruli retain structural relationships between component cell types and can be cultured ex vivo for a short time.
