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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
JoVE Journal
Medicina
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JoVE Journal Medicina
An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

13,021 Views

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10:14 min

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November 01, 2018

DOI:

10.3791/58162-v

DOI:
10.3791/58162-v

•

10:14 min
November 01, 2018

•

2 Views
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1Division of Renal-Electrolyte, Department of Medicine,University of Pittsburgh, 2Department of Cell Biology,University of Pittsburgh

Summary

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The main focus of this protocol is to efficiently isolate viable primary glomeruli cultures with minimal contaminants for use in a variety of downstream applications. The isolated glomeruli retain structural relationships between component cell types and can be cultured ex vivo for a short time.

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Cite this Article

Rush, B. M., Small, S. A., Stolz, D. B., Tan, R. J. An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney. J. Vis. Exp. (141), e58162, doi:10.3791/58162 (2018).

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