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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
Journal JoVE
Médecine
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Journal JoVE Médecine
An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

DOI:
10.3791/58162-v

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10:14 min

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November 01, 2018

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, , ,
1Division of Renal-Electrolyte, Department of Medicine,University of Pittsburgh, 2Department of Cell Biology,University of Pittsburgh

Chapitres

  • 00:04Titre
  • 01:05Isolation of Rat Kidneys
  • 02:17Kidney Tissue Mincing
  • 03:18Kidney Tissue Sieving
  • 07:12Results: Assessment of Efficient Isolation and Cell Viability of Isolated Glomeruli
  • 08:59Conclusion

Summary

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The main focus of this protocol is to efficiently isolate viable primary glomeruli cultures with minimal contaminants for use in a variety of downstream applications. The isolated glomeruli retain structural relationships between component cell types and can be cultured ex vivo for a short time.

Tags

GlomeruliKidney DiseaseFiltration BarrierProteinuric Chronic Kidney DiseaseEuthanized RatHank’s Buffered Salt SolutionKidney Cortex180 Micrometer Sieve

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