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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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JoVE Journal Biyokimya
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

14,783 Views

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08:07 min

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September 11, 2018

DOI:

10.3791/58076-v

DOI:
10.3791/58076-v

•

08:07 min
September 11, 2018

•

17 Views
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1Department of Adaptive Biotechnologies, Global Change Research Institute,Czech Academy of Sciences, 2Department of Plant Physiology, Faculty of Science,Masaryk University, 3Laboratory of Intracellular Regulation, Institute of Plant Physiology,Russian Academy of Sciences

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Here, we present a protocol to quantitatively determine phycobiliprotein content in the cyanobacterium Synechocystis using a spectrophotometric method. The extraction procedure was also successfully applied to other cyanobacteria and algae strains; however, due to variations in pigment absorption spectra, it is necessary to test the spectrophotometric equations for each strain individually.

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Cite this Article

Zavřel, T., Chmelík, D., Sinetova, M. A., Červený, J. Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis. J. Vis. Exp. (139), e58076, doi:10.3791/58076 (2018).

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