Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
12,866 Views
•
14:23 min
•
June 06, 2018
DOI:
Özet
Automatically generated
Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens.
Read Article
Cite this Article
Peterson, T. A., Stamnes, M. A., Piper, R. C. A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions. J. Vis. Exp. (136), e57801, doi:10.3791/57801 (2018).
Copy