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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

12,866 Views

14:23 min

June 06, 2018

DOI:

14:23 min
June 06, 2018

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Summary

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Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens.

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