Journal
/
/
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

12,866 Views

14:23 min

June 06, 2018

DOI:

14:23 min
June 06, 2018

10 Views
, ,

Summary

Automatically generated

Batch processing of yeast 2-hybrid screens allows for direct comparison of the interaction profiles of multiple bait proteins with a highly complex set of prey fusion proteins. Here, we describe refined methods, new reagents, and how to implement their use for such screens.

Read Article