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Caratterizzazione delle glicoproteine con la piega di immunoglobulina mediante cristallografia a raggi x e tecniche biofisiche
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
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Caratterizzazione delle glicoproteine con la piega di immunoglobulina mediante cristallografia a raggi x e tecniche biofisiche

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08:58 min

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July 05, 2018

DOI:

10.3791/57750-v

DOI:
10.3791/57750-v

•

08:58 min
July 05, 2018

•

8 Views
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1Program in Molecular Medicine,The Hospital for Sick Children Research Institute, 2Department of Biochemistry,University of Toronto, 3Department of Immunology,University of Toronto

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Vi presentiamo gli approcci per la caratterizzazione biofisica e strutturale delle glicoproteine con la piega di immunoglobulina biolayer interferometria, calorimetria isotermica di titolazione e cristallografia a raggi x.

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GlycoproteinsImmunoglobulin FoldX-ray CrystallographyBiophysical TechniquesProtein FunctionN-linked GlycansAntibioticsStructural CharacterizationProtein PurificationSize Exclusion ChromatographyDeglycosylationProtein ConcentrationCrystallization

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Ereño-Orbea, J., Sicard, T., Cui, H., Akula, I., Julien, J. Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques. J. Vis. Exp. (137), e57750, doi:10.3791/57750 (2018).

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