Dergi
/
Biyokimya
/
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
JoVE Journal
Biyokimya
This content is Free Access.
JoVE Journal Biyokimya
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

12,247 Views

•

08:58 min

•

July 05, 2018

DOI:

10.3791/57750-v

DOI:
10.3791/57750-v

•

08:58 min
July 05, 2018

•

8 Views
, , , ,
1Program in Molecular Medicine,The Hospital for Sick Children Research Institute, 2Department of Biochemistry,University of Toronto, 3Department of Immunology,University of Toronto

Özet

Automatically generated

We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography.

Explore More Videos

GlycoproteinsImmunoglobulin FoldX-ray CrystallographyBiophysical TechniquesProtein FunctionN-linked GlycansAntibioticsStructural CharacterizationProtein PurificationSize Exclusion ChromatographyDeglycosylationProtein ConcentrationCrystallization

Read Article

Cite this Article

Ereño-Orbea, J., Sicard, T., Cui, H., Akula, I., Julien, J. Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques. J. Vis. Exp. (137), e57750, doi:10.3791/57750 (2018).

Download .ris file

Copy

Share Video

.

Copy