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生物化学
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Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

DOI:
10.3791/57750-v

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08:58 min

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July 05, 2018

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, , , ,
1Program in Molecular Medicine,The Hospital for Sick Children Research Institute, 2Department of Biochemistry,University of Toronto, 3Department of Immunology,University of Toronto

Chapters

  • 00:05Title
  • 00:38Purification of Soluble Glycoprotein from HEK293 Supernatant
  • 02:08Crystallization of Glycoproteins
  • 03:44Soaking Glycoprotein Crystals with its Ligand
  • 04:33Characterization of Fab and Small Molecule Binding to the Glycoprotein
  • 07:34Results: Characterization of Glycoproteins
  • 08:24Conclusion

Summary

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We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography.

Tags

Keywords: GlycoproteinsImmunoglobulin FoldX-ray CrystallographyBiophysical TechniquesProtein FunctionN-linked GlycansAntibioticsStructural CharacterizationProtein PurificationSize Exclusion ChromatographyDeglycosylationProtein ConcentrationCrystallization

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