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Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

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08:58 min

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July 05, 2018

DOI:

10.3791/57750-v

DOI:
10.3791/57750-v

•

08:58 min
July 05, 2018

•

8 Views
, , , ,
1Program in Molecular Medicine,The Hospital for Sick Children Research Institute, 2Department of Biochemistry,University of Toronto, 3Department of Immunology,University of Toronto

Summary

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We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography.

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GlycoproteinsImmunoglobulin FoldX-ray CrystallographyBiophysical TechniquesProtein FunctionN-linked GlycansAntibioticsStructural CharacterizationProtein PurificationSize Exclusion ChromatographyDeglycosylationProtein ConcentrationCrystallization

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Cite this Article

Ereño-Orbea, J., Sicard, T., Cui, H., Akula, I., Julien, J. Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques. J. Vis. Exp. (137), e57750, doi:10.3791/57750 (2018).

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