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Isolatie, karakterisering en High Throughput extracellulaire Flux-analyse van muis primaire renale tubulaire epitheliale cellen
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Isolatie, karakterisering en High Throughput extracellulaire Flux-analyse van muis primaire renale tubulaire epitheliale cellen

DOI:

09:40 min

June 20, 2018

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Bölümler

  • 00:04Başlık
  • 00:50Preparing Collagen I Plates
  • 01:31Perfusion, Digestion and Harvesting Kidneys from Mice
  • 02:36Tissue Processing and Primary Tubular Cells Isolation
  • 04:11Mitochondria Bioenergetics Assay
  • 07:26Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
  • 09:04Conclusion

Özet

Otomatik Çeviri

Dit protocol biedt een rendabele aanpak om te isoleren en karakteriseren van muis primaire renale tubulaire cellen die vervolgens sub kunnen worden gekweekt om te beoordelen renale biologische functies- ex vivo, met inbegrip van de mitochondriale Bioenergetica.

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