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小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析

JoVE Journal
Biyoloji

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JoVE Journal Biyoloji

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

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小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析

16,484 Views

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09:40 min

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June 20, 2018

DOI:

10.3791/57718-v

DOI:

10.3791/57718-v

•

09:40 min

June 20, 2018

•

15 Views

Wen Ding^1,2, Keyvan Yousefi^1,2, Lina A. Shehadeh^2,3,4,5

¹Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, ²Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, ³Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, ⁴Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, ⁵Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine

Özet

该协议提供了一种经济高效的方法, 以分离和表征小鼠原发性肾管细胞, 随后可以进行亚培养, 以评估肾脏生物学功能, 包括线粒体生物能学。

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