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Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
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Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

DOI:
10.3791/57718-v

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09:40 min

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June 20, 2018

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, ,
1Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine

Bölümler

  • 00:04Başlık
  • 00:50Preparing Collagen I Plates
  • 01:31Perfusion, Digestion and Harvesting Kidneys from Mice
  • 02:36Tissue Processing and Primary Tubular Cells Isolation
  • 04:11Mitochondria Bioenergetics Assay
  • 07:26Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
  • 09:04Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This protocol provides a cost-effective approach to isolate and characterize mouse primary renal tubular cells that can subsequently be sub-cultured to assess renal biological functions ex vivo, including mitochondrial bioenergetics.

Etiketler

Renal MetabolismFatty Acid OxidationRenal FibrosisNephropathiesMitochondrial BioenergeticsRenal Epithelial CellsHigh-throughput ScreeningRenal Metabolic DiseasePrimary Renal Tubular Epithelial CellsCollagen-coated DishesMouse Kidney PerfusionKidney DigestionTubular Cell Isolation

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