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Rare Event Detection Using Error-corrected DNA and RNA Sequencing
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August 03, 2018
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Next-generation sequencing (NGS) is a powerful tool for genomic characterization that is limited by the high error rate of the platform (~0.5–2.0%). We describe our methods of error-corrected sequencing that allow us to obviate the NGS error rate and detect mutations at variant allele fractions as rare as 0.0001.
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Wong, W. H., Tong, R. S., Young, A. L., Druley, T. E. Rare Event Detection Using Error-corrected DNA and RNA Sequencing. J. Vis. Exp. (138), e57509, doi:10.3791/57509 (2018).
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