Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Wing H. Wong; R. Spencer Tong; Andrew L. Young; Todd E. Druley

doi:10.3791/57509

Journal

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Genética

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Rare Event Detection Using Error-corrected DNA and RNA Sequencing

JoVE Revista
Genética

This content is Free Access.

JoVE Revista Genética

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

11,635 Views

•

10:36 min

•

August 03, 2018

DOI:

10.3791/57509-v

DOI:

10.3791/57509-v

•

10:36 min

August 03, 2018

•

4 Views

Wing H. Wong*^1,2, R. Spencer Tong*^1,2, Andrew L. Young^1,2, Todd E. Druley^1,2

¹Department of Pediatrics, Division of Hematology and Oncology,Washington University School of Medicine, ²Center for Genome Sciences and Systems Biology,Washington University School of Medicine

Summary

Next-generation sequencing (NGS) is a powerful tool for genomic characterization that is limited by the high error rate of the platform (~0.5–2.0%). We describe our methods of error-corrected sequencing that allow us to obviate the NGS error rate and detect mutations at variant allele fractions as rare as 0.0001.

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Rare Event Detection Using Error-corrected DNA and RNA Sequencing

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