Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Wing H. Wong; R. Spencer Tong; Andrew L. Young; Todd E. Druley

doi:10.3791/57509

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遺伝学

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Rare Event Detection Using Error-corrected DNA and RNA Sequencing

JoVE Journal
遺伝学

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JoVE Journal 遺伝学

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

11,635 Views

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10:36 min

•

August 03, 2018

DOI:

10.3791/57509-v

DOI:

10.3791/57509-v

•

10:36 min

August 03, 2018

•

4 Views

Wing H. Wong*^1,2, R. Spencer Tong*^1,2, Andrew L. Young^1,2, Todd E. Druley^1,2

¹Department of Pediatrics, Division of Hematology and Oncology,Washington University 医学部, ²Center for Genome Sciences and Systems Biology,Washington University 医学部

概要

Next-generation sequencing (NGS) is a powerful tool for genomic characterization that is limited by the high error rate of the platform (~0.5–2.0%). We describe our methods of error-corrected sequencing that allow us to obviate the NGS error rate and detect mutations at variant allele fractions as rare as 0.0001.

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Rare Event Detection Using Error-corrected DNA and RNA Sequencing

概要

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