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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
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Genetik
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JoVE Journal Genetik
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

DOI:
10.3791/57386-v

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09:00 min

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May 02, 2018

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, ,
1Department of Animal Health,Universidad Complutense de Madrid, 2Visavet Health Surveillance Centre,Universidad Complutense de Madrid, 3Department of Zoology,University of Oxford, 4Department of Microbiology,Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

Bölümler

  • 00:05Başlık
  • 00:33Construction of the Experimental System Encoding the Antibiotic Resistance Gene
  • 04:19Evolutionary Rescue Approach to Experimentally Evolve Antibiotic Resistance
  • 06:14Molecular Basis of the Evolution of Antibiotic Resistance
  • 07:05Results: Study of Evolutionary Rescue and Molecular Basis Underlying the Plasmid Mediated Evolution using DNA Sequencing
  • 08:35Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we present an experimental method to test the role of multicopy plasmids in the evolution of antibiotic resistance.

Etiketler

Antibiotic ResistanceMulticopy PlasmidsExperimental MethodMikrobiyolojiBacterial EvolutionMoleküler BiyolojiPCRIsothermal AssemblyElectroporationAntibiotic SelectionDNA SequencingPlasmid Construction

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