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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
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JoVE 杂志 遗传学
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

11,315 Views

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09:00 min

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May 02, 2018

DOI:

10.3791/57386-v

DOI:
10.3791/57386-v

•

09:00 min
May 02, 2018

•

6 Views
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1Department of Animal Health,Universidad Complutense de Madrid, 2Visavet Health Surveillance Centre,Universidad Complutense de Madrid, 3Department of Zoology,University of Oxford, 4Department of Microbiology,Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

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Here we present an experimental method to test the role of multicopy plasmids in the evolution of antibiotic resistance.

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Antibiotic ResistanceMulticopy PlasmidsExperimental MethodMicrobiologyBacterial EvolutionMolecular BiologyPCRIsothermal AssemblyElectroporationAntibiotic SelectionDNA SequencingPlasmid Construction

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Escudero, J. A., MacLean, R. C., San Millan, A. Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance. J. Vis. Exp. (135), e57386, doi:10.3791/57386 (2018).

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