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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

JoVE Journal
Genética

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JoVE Journal Genética

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

11,317 Views

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09:00 min

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May 02, 2018

DOI:

10.3791/57386-v

DOI:

10.3791/57386-v

•

09:00 min

May 02, 2018

•

6 Views

Jose Antonio Escudero^1,2, R Craig MacLean³, Alvaro San Millan⁴

¹Department of Animal Health,Universidad Complutense de Madrid, ²Visavet Health Surveillance Centre,Universidad Complutense de Madrid, ³Department of Zoology,University of Oxford, ⁴Department of Microbiology,Hospital Universitario Ramon y Cajal (IRYCIS) and CIBERESP

Summary

Here we present an experimental method to test the role of multicopy plasmids in the evolution of antibiotic resistance.

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Antibiotic Resistance Multicopy Plasmids Experimental Method Microbiology Bacterial Evolution Biologia Molecular PCR Isothermal Assembly Electroporation Antibiotic Selection DNA Sequencing Plasmid Construction

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